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TZE-CHEN HSIEH, Ph.D.,1,2 JAN KUNICKI, M.S.,2
ZBIGNIEW DARZYNKIEWICZ, M.D., Ph.D.,2 and JOSEPH M. WU, Ph.D.1,2

ABSTRACT

Objective: The goal of this in vitro study was to test the cytostatic and cytotoxic activities of extracts derived from the polysaccharopeptide (PSP), I’m-Yunity,TM (Integrated Chinese Medi- cine Holdings Ltd., Kowloon, Hong Kong) prepared from strain Cov-1 of the mushroom Corio- lus versicolor.

Design: Different volumes of 70% ethanol and water extracts of I’m-Yunity were incubated with cultures of human promyelocytic leukemic HL-60 cells, and compared to nontreated con- trol cells. At various times after treatment, cells were harvested and analyzed with respect to: (1) proliferation and cell cycle phase distribution, (2) induction of apoptosis, and (3) changes in expression of the immunomodulating cytokines interleukin (IL)-1􏰀, IL-6, and IL-8. To test whether extracts also affected normal cells, similar experiments were also performed using iso- lated peripheral blood lymphocytes from healthy volunteers, with and without stimulation by the mitogen phytohemagglutinin (PHA). The ability of extracts to affect the secretion of IL-1􏰀, IL-6, and IL-8 were assessed by enzyme-linked immunosorbent assay.

Results: HL-60 cells incubated with various amounts (1, 3, 5, 7.5, and 10 􏰁l/mL) of the ex- tracts for 1–3 days showed dose-dependent, time-dependent growth suppression and decrease in cell viability. Flow cytometric analysis revealed partial cell arrest in the G1 phase at less than 5 􏰁L/mL and induction of apoptosis at 10 􏰁L/mL or more of ethanol and water extracts, with the latter exhibiting more pronounced inhibition than the former. Experiments performed with lymphocytes demonstrated that extracts of I’m-Yunity alone were without effect; moreover, they also did not affect the lymphocyte response to PHA. Water extract of I’m-Yunity also signifi- cantly increased IL-1􏰀 and IL-6 while substantially lowering IL-8.

Conclusions: I’m-Yunity acts selectively in HL-60 leukemic cells, resulting in cell cycle re- striction through the G1/S checkpoint and the induction of apoptosis.


  1. Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY.
  2. Brander Cancer Research Institute, New York Medical College, Valhalla, NY.

The opinions, views, findings, interpretations and/or recommendations contained in this publication are strictly those of the authors. Trade names of materials and/or products of commercial organizations are cited as needed for precision. These citations do not constitute official endorsement or approval of the use of such commercial materials and/or products.


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